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- HighTherm Reverse Transcriptase 10,000 u (200...
HighTherm Reverse Transcriptase 10,000 u (200 u/uL)
ANG 619
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*All items will import from US
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HighTherm Reverse Transcriptase provides increased specificity and exceptionally high cDNA yields even with complex RNA templates.
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Product Details
- Applications: 1-Step and 2-Step RT-qPCR, Array Labeling, cDNA Libraries, 3' and 5' RACE
- Yield: more representative full-length cDNA due to reduced RNAse H activity (100 bp to > 12 Kb)
- Sensitivity: reproducible cDNA synthesis from a wide range of templates (1 pg to 5ug of total RNA template)
- Thermostability: retains greater than 90% of enzymatic activity following 50°C incubation for 60 minutes
| Brand Name | Azura Genomics |
| Manufacturer | Azura Genomics |
| Included Components | HighTherm™ RT, 5x HighTherm™ Buffer, and 40 mM Ultra-Pure dNTP mix |
| Part Number | AZ-1991 |
| Country of Origin | This item will be imported from US |
| Item Weight | 0.5 lbs (230 grams) |
What's in the box
HighTherm™ RT, 5x HighTherm™ Buffer, and 40 mM Ultra-Pure dNTP mix
Product Description
Customer Questions & Answers
-
Question:
What is the maximum length of cDNA that HighTherm Reverse Transcriptase can synthesize?
Answer: It can synthesize cDNA from 100 bp to over 12 Kb. -
Question:
What types of RNA templates can this enzyme work with?
Answer: It works with ssRNA, ssDNA, and RNA:DNA hybrids. -
Question:
How stable is the enzyme at high temperatures?
Answer: The enzyme retains greater than 90% of its activity following 50°C incubation for 60 minutes.
Azura Genomics Enzymes Editorial Review
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ANG 619
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Features & Benefits
- Engineered for increased specificity and high cDNA yields.
- Functional across a wide temperature range of 38°C to 55°C.
- Efficiently synthesizes cDNA from ssRNA, ssDNA, or RNA:DNA hybrids.
- Significantly reduced Ribonuclease H activity for better yield.
- Supports applications like RT-qPCR, cDNA libraries, and RACE.
- Retains >90% activity after 50°C incubation for 60 minutes.
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