HiMedia Laboratories GM1544-500G Granulated King's Medium B Base, 500 g
ANG 301
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Granulated King's Medium B Base is for the nonselective isolation, cultivation, and pigment production of Pseudomonas species.
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What Stands Out
Product Details
- Suspend 42.23 grams ( the equivalent weight of dehydrated medium per liter) in 1000 ml distilled water containing 15 ml of glycerol
- Heat to boiling to dissolve the medium completely
- Cream to yellow colored granular medium
| Item Form | Granule |
| Item Weight | 500 Grams |
| Brand Name | HiMedia |
| Manufacturer | HiMedia Laboratories, LLC |
| Model Number | GM1544-500G |
| Part Number | GM1544-500G |
| Unit Count | 1.0 Count |
| Brand | HiMedia |
Who Should Buy?
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Microbiology Labs
Ideal for microbiological media preparation, supporting various bacterial growth and species differentiation.
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Research Institutions
Beneficial for academic and industrial research requiring a reliable base for microbial cultures.
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Clinical Applications
Useful in clinical settings to culture and identify pathogens from patient samples effectively.
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Home Users
Not suitable for personal use as it requires specialized knowledge for appropriate application and safety.
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Non-Scientific Settings
Inappropriate for environments lacking the infrastructure for microbial cultivation or monitoring.
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Casual Experimentation
Not recommended for hobbyists or casual users lacking proper training and lab facilities.
Product Description
Customer Questions & Answers
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Question:
What is HiMedia GM1544-500G Granulated King's Medium B Base used for?
Answer: HiMedia GM1544-500G Granulated King's Medium B Base is primarily used for the cultivation of microorganisms, particularly bacteria and fungi. It serves as a rich nutrient medium that supports the growth and maintenance of various microbial species due to its balanced composition of peptones, carbohydrates, and inorganic salts. In practical terms, it's essential for laboratories conducting microbiological research, quality control testing in industries, and educational institutions for teaching purposes. -
Question:
What is the composition of King's Medium B Base?
Answer: King's Medium B Base consists of essential nutrients designed to promote optimal growth of a broad range of microorganisms. Its formulation includes peptones, which provide amino acids and nitrogen, while its carbohydrate content serves as an energy source. This balanced mixture ensures that the cultured organisms thrive. Researchers often choose this medium for isolating and identifying microorganisms in clinical and environmental samples due to its specificity and efficacy. -
Question:
How should I prepare the HiMedia GM1544 Granulated King's Medium B Base?
Answer: To prepare King's Medium B Base, it's generally recommended to dissolve the appropriate amount of granulated medium in distilled water. You typically need to adjust the pH if necessary and sterilize the prepared medium through autoclaving. This temperature and pressure treatment ensures that all contaminating organisms are eliminated. Once prepared, the medium can be cooled and poured into petri dishes or used in slants for culture storage. This careful preparation is crucial for reliable experimental results. -
Question:
Can I store Granulated King's Medium B Base? If so, how?
Answer: Yes, Granulated King's Medium B Base can be stored effectively to prolong its shelf life. It's best to keep it in a cool, dry place, away from direct sunlight and moisture. Typically, closed containers can maintain medium stability for several months. For long-term storage, you can refrigerate it but avoid freezing it to prevent changes in texture and properties. Proper storage is vital for maintaining optimal conditions for microbial studies. -
Question:
Is HiMedia's Granulated King's Medium B Base suitable for clinical microbiology?
Answer: Absolutely, HiMedia's Granulated King's Medium B Base is commonly utilized in clinical microbiology for the isolation and identification of specific bacterial strains, particularly Pseudomonas aeruginosa. Laboratory technicians find this medium advantageous during the culturing process to support screening for infections and antibiotic susceptibility testing. Its specific formulation provides a favorable environment for these pathogens, making it a key resource in diagnostic laboratories. -
Question:
Can King's Medium B Base support anaerobic bacteria growth?
Answer: King's Medium B Base is not specifically designed for the growth of anaerobic bacteria, as it typically requires aerobic conditions to function optimally. However, some anaerobes may still grow under specific alterations or when supplemented with additional reducing agents. Researchers often pair this medium with anaerobic incubation techniques to increase its versatility in cultivation. Thus, it’s essential to assess the specific needs of the bacteria being cultured. -
Question:
What type of packaging does HiMedia GM1544-500G come in?
Answer: HiMedia GM1544-500G Granulated King's Medium B Base typically comes in a resealable, recyclable plastic bag or container that maintains its integrity and prevents contamination. This packaging is designed for laboratory environments, ensuring that the medium remains free from moisture and other contaminants until it is ready for use. Proper packaging helps preserve the quality of the granules, ensuring reliable performance during microbial cultivation. -
Question:
Is the Granulated King's Medium B Base animal-derived?
Answer: HiMedia's Granulated King's Medium B Base is formulated without direct inclusion of animal-derived components, making it suitable for certain applications where such ingredients must be avoided. Its formulation is based on synthetic nutrients, ensuring a consistent and defined medium for microbial growth. This characteristic is especially important in laboratories focusing on producing results applicable to both veterinary and human health research. -
Question:
How does temperature affect microbial growth in King's Medium B Base?
Answer: Temperature plays a crucial role in the growth rate of microorganisms in King's Medium B Base. Typically, a temperature range between 30°C to 37°C is optimal for many bacteria, whereas fungi may thrive at lower temperatures. Adjusting the incubation temperature can lead to significant variations in microbial density and metabolic activity, influencing both qualitative and quantitative experimental outcomes. Researchers often monitor and adjust environmental conditions based on the specific needs of the microorganisms being studied. -
Question:
Where can I buy HiMedia Laboratories GM1544-500G Granulated King's Medium B Base, 500 g?
Answer: You can buy HiMedia Laboratories GM1544-500G Granulated King's Medium B Base, 500 g from Ubuy in Sint Maarten. Ubuy offers a reliable shopping experience, ensuring that you can conveniently purchase this specific medium online and have it delivered to your location.
HiMedia Analytical Reagents GM1544-500G Editorial Review
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Pros
- High-quality granulated medium
- consistent results in microbiology
- Easy to prepare and use
- Excellent for bacterial growth
- Stable formulation for long shelf life
Cons
- Occasional clumping in storage
Product Price History
Important information
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Features & Benefits
- Specially designed for Pseudomonas species.
- Used for nonselective isolation and cultivation.
- Facilitates pigment production.
- Easy preparation by suspending in water and glycerol.
- Boil to completely dissolve the medium.
- Cream to yellow colored granular medium.