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High-Performance Capillary Electrophoresis: Theory, Techniques, and Applications (Chemical Analysis: A Series of Monographs on Analytical Chemistry
ANG 792
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High Performance Capillary Electrophoresis brings together in one volume essential coverage of the theory, techniques, and applications of this highly useful and efficient technology.
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- A complete and up-to-date manual on HPCE theory and practice High Performance Capillary Electrophoresis brings together in one volume essential coverage of the theory, techniques, and applications of this highly useful and efficient technology. Suitable for the novice as well as the experienced user of HPCE, this book features expert contributions from highly respected scientists representing a wide range of disciplines. Chapters, which are grouped into sections to make information easy to find, cover: * Theory and principles of the six HPCE techniques * Detection systems, including indirect detection * Essential operation topics such as sample introduction and stacking, coated capillaries, and method validation * Recently developed methods, including two-dimensional separations, nonaqueous CE, and HPCE on microchips * All of the basic HPCE applications, with an emphasis on bioanalytical uses * HPCE in the determination of physico-chemical properties of molecules With features and capabilities that match--and even surpass--those of conventional electrophoresis and HPLC, high performance capillary electrophoresis (HPCE) is the fastest developing technology for the separation and analysis of chemical compounds. Keeping pace with the rapid changes in this field and the wealth of journal articles on the subject is a difficult and time-consuming challenge for anyone needing a basic and up-to-date grasp of HPCE. This book makes it much easier to find this important information--with comprehensive one-source coverage of all of the essential aspects of HPCE theory, techniques, and applications. Featuring the contributions of well-qualified, highly regarded scientists, it is organized into sections on: * Theory and principles of HPCE techniques * Detection systems * Operation aspects and special methods in HPCE * Uses in chemical analysis * Physico-chemical studies Specific topics addressed here that are not treated extensively by other books include two-dimensional separations, CE on microchips, nonaqueous CE, indirect detection, monitoring enzymatic reactions, and more. As interest in HPCE continues to grow, it is clear that this technology has much to offer researchers and others working in disciplines ranging from analytical chemistry and biochemistry to pharmaceutical chemistry and biotechnology. High Performance Capillary Electrophoresis equips scientists and students with the knowledge they need to take immediate advantage of the exciting potential of HPCE.
| Publisher | Wiley-Interscience |
| Publication date | April 27, 1998 |
| Edition | 1st |
| Language | English |
| Print length | 1080 pages |
| ISBN-10 | 0471148512 |
| ISBN-13 | 978-0471148517 |
| Item Weight | 3.5 pounds (1.59 kg) |
| Dimensions | 6.34 x 2.15 x 9.21 inches (16.1 x 5.5 x 23.4 cm) |
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Who Should Buy?
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Academic Researchers
Ideal for researchers needing comprehensive insights into capillary electrophoresis theory and diverse applications in analytical chemistry.
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Laboratory Professionals
Perfect for lab technicians seeking to enhance their skills in high-performance capillary electrophoresis techniques and best practices.
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Graduate Students
Beneficial for graduate students studying analytical chemistry, providing foundational knowledge and practical applications of electrophoresis.
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Beginner Users
Not suitable for beginners without prior knowledge of electrophoresis or analytical chemistry concepts and methods.
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General Audience
Not recommended for casual readers or those seeking general knowledge without a focus on specialized scientific techniques.
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Budget-Conscious Buyers
May not fit users looking for inexpensive resources, as specialized academic texts typically come at a higher price point.
Product Description
Customer Questions & Answers
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Question:
What is High-Performance Capillary Electrophoresis (HPCE)?
Answer: High-Performance Capillary Electrophoresis (HPCE) is an advanced analytical technique utilized for the separation of ionic species by their charge and size. In essence, HPCE leverages an electric field to drive charged molecules through a capillary tube filled with an electrolyte. This method is highly efficient and allows for rapid analysis, making it ideal for various applications, including pharmaceutical research and environmental analysis. Researchers often choose HPCE over traditional methods because of its high resolution and minimal sample requirements. -
Question:
What are the primary benefits of using HPCE?
Answer: The main benefits of High-Performance Capillary Electrophoresis include its exceptional separation efficiency, speed of analysis, and lower sample consumption. Unlike other chromatographic techniques, HPCE can separate complex mixtures in a fraction of the time due to its reduced diffusion. Additionally, it is particularly useful in the analysis of small molecules, proteins, and nucleic acids, expanding its applicability in fields like biochemistry and pharmaceuticals. This makes HPCE a vital tool for scientists and researchers aiming for precise and quick results. -
Question:
What are the main applications of HPCE in chemical analysis?
Answer: HPCE is employed in a variety of applications across different fields, including pharmaceuticals, environmental testing, and food safety. In pharmaceutical research, it helps in analyzing drug formulations and metabolites, providing insights into stability and efficacy. For environmental scientists, HPCE facilitates the detection of contaminants in water and soil samples. Additionally, in food safety, it is used to identify and quantify additives and residuals. The versatility of HPCE makes it an essential technique in modern analytical chemistry. -
Question:
How does the capillary electrophoresis technique differ from traditional electrophoresis methods?
Answer: Capillary electrophoresis differentiates itself from traditional electrophoresis techniques primarily in scale and efficiency. Unlike traditional methods that use larger gels or slabs, capillary electrophoresis utilizes a narrow capillary tube. This design minimizes heat generated during operation, allowing for higher electric fields and faster separations. Consequently, results can be obtained in a significantly shorter time frame, which is crucial for high-throughput laboratories. This innovation is particularly beneficial for labs handling diverse and complex samples. -
Question:
What types of samples can be analyzed using HPCE?
Answer: HPCE can analyze a wide range of sample types, including biological fluids, pharmaceuticals, food products, and environmental samples. Biological samples such as plasma and urine are often analyzed to monitor drug levels and metabolites. In pharmaceutical development, HPCE is used for stability testing of drug formulations. Moreover, its application extends to food analysis, where it can detect additives and contaminants, ensuring safety and compliance. The broad applicability makes HPCE a versatile choice for researchers in various domains. -
Question:
What are the limitations of High-Performance Capillary Electrophoresis?
Answer: While High-Performance Capillary Electrophoresis offers numerous advantages, it also has some limitations. One of the primary challenges is its sensitivity to buffer composition and environmental conditions, which can affect reproducibility. Additionally, HPCE may not be suitable for very large biomolecules that do not migrate efficiently through the capillary. Furthermore, specialized knowledge and training may be required to operate the equipment effectively and interpret results accurately. Understanding these limitations is crucial for optimal application in research. -
Question:
What is the significance of buffer systems in HPCE?
Answer: Buffer systems play a crucial role in High-Performance Capillary Electrophoresis as they directly influence the electroosmotic flow and the separation efficiency of analytes. The choice of buffer can affect the solubility, ionic strength, and overall interaction of molecules within the capillary. For effective separation, an appropriate buffer system ensures optimal pH and conductivity, enhancing resolution and stability of the separation process. Researchers often experiment with various buffer compositions to achieve the desired separation outcomes in complex mixtures. -
Question:
Can HPCE be used for protein analysis?
Answer: Yes, High-Performance Capillary Electrophoresis is widely used for protein analysis. Its ability to separate proteins based on their charge and size makes it an invaluable tool in proteomics. Researchers utilize HPCE to analyze protein purity, identify isoforms, and study post-translational modifications. This technique is particularly advantageous because it requires minimal sample volume and provides rapid results, making it ideal for studies where sample quantity may be limited. HPCE thus plays a critical role in advancing our understanding of protein functionality. -
Question:
What are the typical detection methods used with HPCE?
Answer: Common detection methods used alongside High-Performance Capillary Electrophoresis include ultraviolet (UV) absorption, fluorescence, and mass spectrometry. UV detection is popular due to its simplicity and efficiency in monitoring various analytes. Fluorescence detection enhances sensitivity for specific compounds, making it useful for trace analysis. Additionally, coupling HPCE with mass spectrometry allows for sophisticated analysis of molecular weight and structural information, further expanding its utility in research. Each detection method contributes uniquely to the overall applicability of HPCE in analytical chemistry. -
Question:
Where can I buy High-Performance Capillary Electrophoresis: Theory, Techniques, and Applications Chemical Analysis?
Answer: You can purchase 'High-Performance Capillary Electrophoresis: Theory, Techniques, and Applications' from Ubuy. Ubuy offers a variety of scientific books and publications, including those focused on analytical chemistry and advanced techniques. This platform is renowned for its extensive selection, ensuring that you can find resources essential for your research or academic needs. By shopping on Ubuy, you can conveniently explore related products and materials that enhance your understanding of capillary electrophoresis and its applications.
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Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
Features & Benefits
- Comprehensive coverage of theory, techniques, and applications of HPCE
- Suitable for novice and experienced users
- Expert contributions from highly respected scientists
- Covers theory, detection systems, operation aspects, and uses
- Includes topics not extensively covered in other books
- Equips scientists and students to take immediate advantage of HPCE
